A Comparative Analyzing of Zooplankton Community Diversity in Surface Layer Water of Reservoir Via eDNA Metabarcoding and Microscopy

We compared two sampling methods, eDNA metabarcoding and microscope identification (MSI), for the analysis of zooplankton diversity in reservoirs with its inflow outflow streams. The dynamic patterns Cladocera Rotifera at different time points were similar between but there was a slight difference Copepoda. Specifically, members Copepoda subclass could not be easily classified using MSI method, whereas detect minor taxa Rotifera. Upon comparing list communities Korea gene database NCBI, only ~56% genera reported detected based on 18S rRNA gene. However, more diverse range despite lack genetic information. As water temperature increased after May, decreased according to method method. Although has some limitations, it able wider MSI. provides reliable means identify zooplankton.